Mafruha AfrozBangladesh Agricultural Research Institute, India
Title: Differential colonization of Solanum sisymbriifolium by Ralstonia solanacearum strains
Grafting desired scions of tomato and eggplant onto disease-resistant rootstocks is a tactic used to manage bacterial wilt caused by Ralstonia solanacearum in South Asia. Solanum sisymbriifolium is frequently used as a rootstock, but plants grafted onto this species have failed recently in some locations in Bangladesh and Nepal. To determine the susceptibility of S. sisymbriifolium plants to colonization by R. solanacearum, eight strains from wilted tomato or eggplant grafted onto S. sisymbriifolium rootstocks, or wilted non-grafted plants in Bangladesh and Nepal were used to challenge S. sisymbriifolium seedlings.. Plants were inoculated by root drenching and stems were cut 0.5 cm above the soil line 5, 12 and 20 days post-inoculation (dpi), then placed in 500 µl sterilized distilled water for 24 hrs. Populations of R. solanacearum in the bacterial ooze determined by quantitative real-time PCR (qPCR) were strongly positively correlated with populations determined by dilution plating. All eight strains were recovered from inoculated plants. R. solanacearum populations remained relatively low until 20 dpi. There were significant differences in numbers of bacteria detected in ooze among the eight strains. None of the S. sisymbriifolium plants wilted up to 36 dpi, although tomato ‘OH7814’ seedlings inoculated with the same R. solanacearum strains began wilting at 7 dpi.
DR. Mafruha Afroz currently works as a Senior Scientific Officer in Plant Pathology, Horticulture Research Center, Bangladesh Agricultural Research Institute, Joydevpur, Gazipur. My current project is Disease Management of Horticultural Crops. My special area is Bacteriology. I am interested to Plant Disease diagnosis by using all kinds of molecular techniques such as PCR, Multiplex PCR, Real Time PCR, BOX/REP PCR, MLTS etc. which I learned from Professor Dr. Sally Miller's Lab, Ohio State University, USA during my doctoral program and control the Plant Disease.