According to emergence of resistance bacteria to antibiotics in the food industry, the application of bacteriophages may be a solution due to their specific function on goal bacteria. In this study, composite film of gelatin-pullulan loaded with bacteriophages was prepared, and mechanical, water vapor permeability, and transparency properties of film was investigated. As well as, its anti-bacterial activity against Salmonella typhimurium and Salmonella enteritidis on agar media and meat was studied. The results showed that incorporation of phages into film increased elongation at break and transparency, also water vapor permeability did not have significant changing. Anti-bacterial activity of film on the media agar and beef meat at 11 temperature against Salmonella typhimurium were higher than Salmonella enteritidis. Population of S. typhimurium and S. enteritidis on the beef meat decreased 1.5 log unit (CFU/ml) and 0.8 log unit (CFU/ml) after 24 h compare to control groups, respectively. This edible film containing phages as a bio-control approach can improve quality and food safety on the matrix food against Salmonella pathogen bacteria, especially since it has a specific action