Elena A. ZverevaMoscow, Russian Academy of Sciences, Russia
Title : Development of lateral flow assays to control composition of meat products: Characterization of different biomarkers
Rapid monitoring of the composition of meat products is extremely demanded in modern society. Lateral flow assay (LFA) allows efficient solving these tasks. The method is based on the use of a multimembrane composite, on which all reagents are preliminarily applied. This construction provides simple and rapid testing directly at the sampling sites, but needs in special adoption to identify animal species and tissues. The presented study was financially supported by the Russian Science Foundation (Grant 19-16-00108) and focused on the comparison of different biomarkers having protein and nucleic acid nature.
Correspondingly, the first proposed type of LFA tests was based on protein biomarkers recognition using specific antibodies. The selected biomarkers (skeletal troponin I, myoglobin, mammalian IgG, avian IgY) were characterized by a stable high content in muscle tissues, interspecies variation of antigenic properties and their retention under various types of industrial processing. The second type of tests is based on the LFA coupled with reverse transcription recombinase polymerase amplification. Primers for PCR identification of porcine and chicken meat were used, and LFA conditions for them were chosen. The developed test-systems allow detecting selected biomarkers with high accuracy, which makes it possible to determine quantitatively the composition of both meat mixtures, and finished meat food products.
Elena A. Zvereva is a senior researcher in A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences. Her scientific interests are in the field of development of new immunochemical techniques for the detection of biologically active compounds – antibiotics, pesticides, hormones, proteins. These works cover the full cycle of the development of immunoanalytical techniques, beginning from reactants obtaining and finalizing by the creation of manufacturing technologies.